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Survival mode characters
Defeat one of the following opponents in Vs. mode to unlock them in Survival mode:
Bazooka Marine: Defeat the Bazooka Marine in the Fossil Beach.
Blue Demon General: Defeat the Blue Demon General in the Cave Zone.
Cluster Demon Soldier: Defeat the Cluster Demon Soldier in the Cave Zone.
Crimson Demon General: Defeat the Crimson Demon General in the Cave Zone.
Knuckle Pirate: Defeat the Knuckle Pirate in the Autumn Colored Ruins.
Marine Corps Chief: Defeat the Marine Corps Chief in the Mystery Jungle.
Normal Marine: Defeat the Normal Marine in the Fossil Beach.
Pistol Pirate: Defeat the Pistol Pirate in the Mystery Jungle.
Saber Pirate: Defeat the Saber Pirate in the Mystery Jungle.
Sword Demon Soldier: Defeat the Sword Demon Soldier in the Cave Zone.
World Government Agent: Defeat the World Government Agent in the Autumn Colored Ruins.
Vs. mode characters
Defeat one of the following opponents in Story mode to unlock them in Vs. mode:
Aokiji: Defeat Aokiji in Land To Heaven.
Arlong: Defeat Arlong in the Cave area in Story mode.
Bon Clay (Mr. 2): Unlock the Orb Spot at Fossil Beach that leads to the optional Red Orb spot, and defeat him.
Calgara: Unlock the Orb Spot at the Mystery Jungle that leads to the optional Red Orb spot, and defeat him.
Crocodile: Defeat Crocodile in the Cave area in Story mode. Note: He is the second Boss. To locate him, hit the wall with a character instead of a pick axe.
Eneru: Defeat Eneru at the Autumn Colored Ruins in Story mode.
Evil Guardian: Defeat the final Boss in Beginning and End.
Evil Master Beast: Defeat the final Boss in Beginning and End.
Kaku: Defeat Kaku in the Jungle area.
Lucci: Defeat Lucci in Land To Heaven.
Mihawk: Defeat Mihawk in the Fossil Beach area in Story mode.
Paulie: Unlock the Orb Spot at the Cave of Darkness that leads to the optional Red Orb spot, and defeat him.
Portgas D. Ace: Defeat Portgas D. Ace in the Silver Mountains.
Rampaging Chopper: Unlock the Orb Spot at Silver Mountains that leads to the optional Red Orb spot, and defeat him.
Rob Lucci: Defeat Rob Lucci in Land To Heaven.
Shanks: Defeat Shanks in the Silver Mountains.
Smoker: Defeat Smoker just before you enter Fossil Beach in Story mode.
Spandam: Defeat Spandam in the Autumn Colored Ruins in Story mode. Note: He is the first Boss.
Vivi: Unlock the Orb Spot at the Autumn Colored Ruins that leads to the optional Red Orb spot, and defeat her.
Whitebeard: Unlock the Orb Spot in the Land to Heaven that leads to the optional Red Orb spot, and defeat him.
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